Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma

作者：Dapeng Yun, Yuqi Wang, Delong Meng, Yuanyuan Ji, Juxiang Chen, Hongyan Chen, Daru Lu · 发表于：Oncology Reports · 年份：2018 · DOI：10.3892/or.2018.6225 · 被引用次数：35 · 研究领域：Cell Image Analysis Techniques、Advanced Electron Microscopy Techniques and Applications、Glioma Diagnosis and Treatment

Glioma is the most aggressive and malignant primary brain tumor in adults. In the present study, we identified a vital oncoprotein, capping actin protein, gelsolin-like (CapG), and investigated its roles in the prognosis, proliferation and metastasis in glioma. The mRNA and protein levels of CapG were significantly increased in human glioma, and higher CapG expression was an independent prognostic factor for predicting unfavorable prognosis. The expression level of CapG was found to be associated with several common molecular features of glioblastoma (GBM; WHO grade IV glioma) in The Cancer Genome Atlas (TCGA) cohort. When analyzing the prognosis of GBM patients according to these molecular features, we observed that the prognostic value of CapG was affected by amplification of CDK6 or EGFR. However, overexpression of CapG markedly promoted cell growth in vitro, while depletion of CapG significantly inhibited cell proliferation by blocking the cell cycle in G1/S transition. Moreover, CapG manipulation in glioma cell lines U87 and U251 showed CapG-dependent cellular migration and invasiveness. These data suggest that CapG may serve as a prognostic biomarker with potentially important therapeutic implications for glioma.