Synthetic Whole-Cell Biodevices for Targeted Degradation of Antibiotics

作者：Peng‐Fei Xia, Qian Li, Lin-Rui Tan, Miao Liu, Yong‐Su Jin, Shu-Guang Wang · 发表于：Scientific Reports · 年份：2018 · DOI：10.1038/s41598-018-21350-9 · 被引用次数：5 · 研究领域：Innovative Microfluidic and Catalytic Techniques Innovation、CRISPR and Genetic Engineering、Microbial Fuel Cells and Bioremediation

Abstract Synthetic biology enables infinite possibilities in biotechnology via employing genetic modules. However, not many researches have explored the potentials of synthetic biology in environmental bioprocesses. In this study, we introduced a genetic module harboring the codon-optimized tetracycline degrading gene, tetX.co , into the model host, Escherichia coli , and generated a prototypal whole-cell biodevice for the degradation of a target antibiotic. Our results suggested that E. coli with the tetX.co- module driven by either the P J23119 or P BAD promoters conferred resistance up to 50 μg/mL of tetracycline and degrades over 95% of tetracycline within 24 h. The detoxification ability of tetX was further verified in conditioned media by typical E. coli K-12 and B strains as well as Shewanella oneidensis . Our strategy demonstrated the feasibility of introducing genetic modules into model hosts to enable environmental functions, and this work will inspire more environmental innovations through synthetic biological devices.