IL ‐33/ ST 2 axis affects the polarization and efferocytosis of decidual macrophages in early pregnancy

作者：Yan‐Ran Sheng, Wenting Hu, Chunyan Wei, Lingli Tang, Yukai Liu, Yu‐Yin Liu, Jianping Qiu, Da‐Jin Li, Xiao‐Yong Zhu · 发表于：American Journal of Reproductive Immunology · 年份：2018 · DOI：10.1111/aji.12836 · 被引用次数：58 · 研究领域：IL-33, ST2, and ILC Pathways、Reproductive System and Pregnancy、Immune cells in cancer

PROBLEM: To explore whether IL-33/ST2 axis modulates the polarization and efferocytosis of decidual macrophages (dMφs). METHOD OF STUDY: The phenotype characteristics of dMφs from both normal pregnant women and recurrent spontaneous abortion (RSA) patients were determined by real-time polymerase chain reaction (RT-PCR) and flow cytometry (FCM). Then, the efferocytosis and expression of IL-33 and its receptor (ST2) in dMφs were analyzed by FCM. Finally, the effects of sST2, a decoy receptor for IL-33 that inhibits the IL-33/ST2 signaling pathway, on the polarization and efferocytosis of dMφs and human macrophage cell line U937 were investigated. RESULTS: Compared with normal pregnancy, dMφs from RSA patients presented a M1 phenotype with high secretion of IL-33, whereas the expression of ST2 decreased. However, dMφs from RSA patients possessed a more powerful efferocytosis ability to clear the apoptotic decidual stromal cells (DSCs) compared with dMφs from normal pregnancy patients. Treatment with recombinant human sST2 led to the up-regulation of M1 bias and efferocytosis ability of both normal dMφs and U937. CONCLUSION: This study indicates that IL-33 secreted by dMφs promotes M2 bias at the feto-maternal interface, and as a result, RSA might attribute to the disturbance of IL-33/ST2 axis and the enhancement of efferocytosis of dMφs subsequently.