Effect of bone marrow mesenchymal stem cells on the polarization of macrophages

作者：Zheng Yuan, Yi Deng, Wen Lai, Zheng Shao, Hui Bian, Liu Zu, Zhi Huang, Chuan Sun, Han Li, Hong Jie Luo, Liang Ma, Han Y. H. Chen, Bing Xiong · 发表于：Molecular Medicine Reports · 年份：2018 · DOI：10.3892/mmr.2018.8457 · 被引用次数：42 · 研究领域：Immune cells in cancer、Inflammation biomarkers and pathways、Mesenchymal stem cell research

Inflammation is a defensive response in the living tissue of the vascular system that acts against damage factors and involves various types of immune cells, including macrophages, neutrophils, endothelial cells and other associated immune molecules. If the release of inflammatory mediators is excessive, systemic inflammatory response syndrome may develop. Sepsis is the most common complication of severe burns and is a systemic inflammatory response syndrome that is caused by infectious factors and is capable of leading to multiple organ dysfunction and potentially death. Research concerning the mechanism and treatment of sepsis is crucial. Macrophages are an important type of immune cell that remove invasive pathogens and are involved in innate and adaptive immune responses. It has been previously reported that bone marrow mesenchymal stem cells (BMSCs) affect macrophages by regulating immunity. The present study aimed to investigate the effect of BMSCs on macrophage polarization in vivo and in vitro, in addition to the potential therapeutic effect of these cells on experimental sepsis. BMSCs and peritoneal macrophages were isolated from Sprague‑Dawley rats and co‑cultured overnight as a mixed culture or Transwell system, and subsequently stimulated with 100 ng/ml lipopolysaccharide (LPS). After 12 h, the medium was replaced with normal complete medium for various durations and supernatants were collected to extract proteins and cells for ELISA, western blot and flow cytomet...