Chikungunya virus nsP1 interacts directly with nsP2 and modulates its ATPase activity

作者：Sameer Kumar, Abhishek Kumar, Prabhudutta Mamidi, Atul Kumar Tiwari, Sriram Kumar, Animamalar Mayavannan, Sagarika Mudulli, Ajit Singh, Bharat Bhusan Subudhi, Soma Chattopadhyay · 发表于：Scientific Reports · 年份：2018 · DOI：10.1038/s41598-018-19295-0 · 被引用次数：31 · 研究领域：Mosquito-borne diseases and control、HIV Research and Treatment、Studies on Chitinases and Chitosanases

Abstract Chikungunya virus (CHIKV) is a mosquito-borne virus, which has created an alarming threat in the world due to unavailability of vaccine and antiviral compounds. The CHIKV nsP2 contains ATPase, RTPase, helicase and protease activities, whereas, nsP1 is a viral capping enzyme. In alphaviruses, the four non-structural proteins form the replication complex in the cytoplasm and this study characterizes the interaction between CHIKV nsP1 and nsP2. It was observed that, both the proteins co-localize in the cytoplasm and interact in the CHIKV infected cells by confocal microscopy and immunoprecipitation assay. Further, it was demonstrated through mutational analysis that, the amino acids 1-95 of nsP2 and 170-288 of nsP1 are responsible for their direct interaction. Additionally, it was noticed that, the ATPase activity of nsP2 is enhanced in the presence of nsP1, indicating the functional significance of this interaction. In silico analysis showed close (≤1.7 Å) polar interaction (hydrogen bond) between Glu 4 , Arg 7, 96, 225 of nsP2 with Lys 256, 206 , Val 367 and Phe 312 of nsP1 respectively. Hence, this investigation provides molecular characterization of CHIKV nsP1-nsP2 interaction which might be a useful target for rational designing of antiviral drugs.