Supramolecular Recognition of Three Way Junction DNA by a Cationic Calix[3]carbazole

作者：Zhaozheng Yang, Yan Chen, Gang Li, Zhangmin Tian, Liang Zhao, Xi Wu, Qi Ma, Mingzhe Liu, Peng Yang · 发表于：Chemistry - A European Journal · 年份：2018 · DOI：10.1002/chem.201705564 · 被引用次数：34 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、Metal complexes synthesis and properties

Abstract DNA three‐way junctions (TWJ‐DNA) are intermediate structures in DNA replication and/or recombination. They play very important roles in biological processes, but more subtle functions are still unknown due partially to the lack of a fluorescent ligand. In this study, a cationic calix[3]carbazole (2) has been synthesized and its properties of interacting with TWJ‐DNA have been evaluated by UV/Vis and fluorescence spectroscopy, circular dichroism (CD), gel electrophoresis, and 1H NMR studies. The results show that 2 binds to the central hydrophobic cavity of TWJ‐DNA. Moreover, it could selectively bind to TWJ‐DNA over duplex and quadruplex DNA. Furthermore, 2 possesses the capability of serving as the TWJ‐DNA probe as its trap‐II excimer emission is turned on by TWJ‐DNA.