Multiplexed Profiling of Single Extracellular Vesicles

作者：Kyungheon Lee, Kyle Fraser, Bassel Ghaddar, Katy Yang, Eunha Kim, Leonora Balaj, E. Antonio Chiocca, Xandra O. Breakefield, Hakho Lee, Ralph Weissleder · 发表于：ACS Nano · 年份：2017 · DOI：10.1021/acsnano.7b07060 · 被引用次数：378 · 研究领域：Extracellular vesicles in disease、MicroRNA in disease regulation、Cell Adhesion Molecules Research

Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (∼4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is simple, sensitive, multiplexable, and practical. We profiled glioblastoma EV and discovered surprising variations in putative pan-EV as well as tumor cell markers on EV. These analyses shed light on the heterogeneous biomarker profiles of EV. The SEA technology has the potential to address fundamental questions in vesicle biology and clinical applications.