Immunomodulatory Effect of Flavonoids of Blueberry (Vaccinium corymbosum L.) Leaves via the NF-κB Signal Pathway in LPS-Stimulated RAW 264.7 Cells

作者：Dazhi Shi, Mengyi Xu, Mengyue Ren, En‐Shan Pan, Chaohua Luo, Wei Zhang, Qingfa Tang · 发表于：Journal of Immunology Research · 年份：2017 · DOI：10.1155/2017/5476903 · 被引用次数：25 · 研究领域：Natural product bioactivities and synthesis、Bioactive natural compounds、NF-κB Signaling Pathways

Objective . This study aimed to explore the immunoregulatory effect of flavonoids of blueberry ( Vaccinium corymbosum L.) leaves (FBL). Methods . The flavonoids of blueberry leaves were prepared with 70% ethanol and were identified by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-Tof-MS). The immunoregulatory effect and possible regulatory mechanisms of FBL were investigated in lipopolysaccharide- (LPS-) induced RAW 264.7 cells. Results . According to the results of UPLC/Q-Tof-MS, nine flavonoids of blueberry leaves were identified. FBL showed a significant reduction in the production of TNF- α in LPS-stimulated RAW 264.7 cells. FBL significantly decreased the expression of NF- κ B p65 and P-NF- κ B p65 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Conclusion . Our study showed the immunoregulatory effect of FBL through the suppression of TNF- α via the NF- κ B signal pathway.