Effects of zinc on the production of alcohol by Clostridium carboxidivorans P7 using model syngas

作者：Demao Li, Chunxiao Meng, Guanxun Wu, Bintao Xie, Yifan Han, Yaqiong Guo, Chunhui Song, Zhengquan Gao, Zhiyong Huang · 发表于：Journal of Industrial Microbiology & Biotechnology · 年份：2017 · DOI：10.1007/s10295-017-1992-2 · 被引用次数：29 · 研究领域：Microbial Metabolic Engineering and Bioproduction、Biofuel production and bioconversion、Catalysis for Biomass Conversion

Abstract Renewable energy, including biofuels such as ethanol and butanol from syngas bioconversed by Clostridium carboxidivorans P7, has been drawing extensive attention due to the fossil energy depletion and global eco-environmental issues. Effects of zinc on the growth and metabolites of C. carboxidivorans P7 were investigated with model syngas as the carbon source. The cell concentration was doubled, the ethanol content increased 3.02-fold and the butanol content increased 7.60-fold, the hexanol content increased 44.00-fold in the medium with 280 μM Zn2+, when comparing with those in the control medium [Zn2+, (7 μM)]. Studies of the genes expression involved in the carbon fixation as well as acid and alcohol production in the medium with 280 μM Zn2+ indicated that fdhII was up-regulated on the second day, acs A, fdhII, bdh35 and bdh50 were up-regulated on the third day and bdh35, acsB, fdhI, fdhIII, fdhIV, buk, bdh10, bdh35, bdh40 and bdh50 were up-regulated on the fourth day. The results indicated that the increased Zn2+ content increased the alcohol production through increase in the gene expression of the carbon fixation and alcohol dehydrogenase.