Correlation between miRNAs and target genes in response to Campylobacter jejuni inoculation in chicken

作者：H. Wang, Li‐Jun Liu, Xu-Zhou Liu, Mengmeng Zhang, X. Li · 发表于：Poultry Science · 年份：2017 · DOI：10.3382/ps/pex343 · 被引用次数：11 · 研究领域：MicroRNA in disease regulation、interferon and immune responses、Aquaculture disease management and microbiota

Campylobacter jejuni (C. jejuni) is one of the main causes of human campylobacteriosis worldwide. Chicken is the main reservoir of C. jejuni. The cecum is the primary site of C. jejuni colonization. Our previous study has shown that the CLOCK gene is important to regulate the response to C. jejuni inoculation and has a cross-talking with immune-related genes. Correlation networks between the CLOCK gene and microRNAs (miRNAs) play vital roles in colorectal tumorigenesis. MiRNAs play crucial roles in bacterial invasion through regulating multiple target genes. To study the regulatory correlation between miRNAs and target genes, 5 miRNAs that regulate the expression of the CLOCK gene (CLOCK-interacted miRNAs) and 5 immune-related target genes predicted through Targetscan and miRDB were selected to analyze the expression pattern in chicken ceca at 4, 8, 12, 16, 20, and 24 h post C. jejuni inoculation using quantitative PCR. The results showed that the expression of gga-miR-148a, gga-miR-1416-5p, gga-miR-30b, and gga-miR-30c were significantly up-regulated at 8 hpi, and gga-miR-30a-5p, gga-miR-30b, and gga-miR-30c were significantly down-regulated at 24 hpi, and gga-miR-1416-5p was significantly down-regulated at 20 hpi (P < 0.05). The expression levels of BCL9 (B-cell CLL/lymphoma 9), STX16 (syntaxin16), IL4R (interleukin 4 receptor), and IRF4 (interferon regulatory factor 4) genes were significantly up-regulated at 8 hpi, while SOCS3 (suppressor of cytokine signaling 3) was sign...