Modulation of inflammation by toll-like receptor 4/nuclear factor-kappa B in diarrhea-predominant irritable bowel syndrome

作者：Xing He, Lihong Cui, Xiaohui Wang, Zhihui Yan, Chao Li, San-Dong Gong, Yan Zheng, Zhe Luo, Ying Wang · 发表于：Oncotarget · 年份：2017 · DOI：10.18632/oncotarget.23045 · 被引用次数：37 · 研究领域：Gastrointestinal motility and disorders、Helicobacter pylori-related gastroenterology studies、Congenital gastrointestinal and neural anomalies

// Xing He 1 , Li-Hong Cui 2 , Xiao-Hui Wang 2 , Zhi-Hui Yan 2 , Chao Li 2 , San-Dong Gong 2 , Yan Zheng 2 , Zhe Luo 2 and Ying Wang 2 1 Third Military Medical University, Chongqing 400038, China 2 Department of Gastroenterology, Navy General Hospital of PLA, Beijing 100048, China Correspondence to: Li-Hong Cui, email: luckycui861@sina.com Keywords: toll-like receptor 4; nuclear factor-kappa B; diarrhea-predominant irritable bowel syndrome; inflammatory cytokines; pyrrolidine dithiocarbamate Received: August 12, 2017     Accepted: November 16, 2017     Published: December 08, 2017 ABSTRACT In order to investigate the function of toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signal pathways in the pathogenesis of diarrhea-predominant irritable bowel syndrome (IBS-D), IBS-D animal models were established in wistar rats challenged with acute and chronic stresses (29 days). Wistar rats without stress-challenged were used as controls. IBS-D models were randomly divided into two groups: one was treated with normal saline, another group was treated with TLR4/NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC) (50mg/kg/week) for continuous four times. Our results demonstrate that continuous stresses can induce the characteristic symptoms of IBS-D, including high wet stool rate and intestinal flora imbalance. Further examinations of colon tissues show that the protein expression levels of TLR4 and NF-κB in IBS-D grou...