The expression of the embryonic gene Cripto‐1 is regulated by OCT 4 in human embryonal carcinoma NCCIT cells

作者：Sung‐Won Park, Hyun‐Jin Do, Mi‐Hee Han, Wonbin Choi, Jae‐Hwan Kim · 发表于：FEBS Letters · 年份：2017 · DOI：10.1002/1873-3468.12935 · 被引用次数：9 · 研究领域：Pluripotent Stem Cells Research、Developmental Biology and Gene Regulation、Genomics and Chromatin Dynamics

Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression is increased by OCT4 overexpression, but decreased by shRNA-mediated OCT4 knockdown. OCT4 overexpression significantly activates Cripto-1 transcriptional activity. A 5'-upstream minimal promoter sequence in the gene-encoding Cripto-1 is significantly activated by OCT4 overexpression. Mutation of the putative OCT4-binding site abolishes OCT4-mediated activation of the Cripto-1 promoter. The OCT4 transactivation domains mediate transcriptional activity of the Cripto-1 minimal promoter through direct interaction. Taken together, OCT4 plays an important role as a transcriptional activator of Cripto-1 expression in NCCIT cells.