Assigning chemoreceptors to chemosensory pathways in Pseudomonas aeruginosa

作者：Davi R. Ortega, Aaron D. Fleetwood, Tino Krell, Caroline S. Harwood, Grant J. Jensen, Igor B. Zhulin · 发表于：Proceedings of the National Academy of Sciences · 年份：2017 · DOI：10.1073/pnas.1708842114 · 被引用次数：82 · 研究领域：Bacterial Genetics and Biotechnology、RNA and protein synthesis mechanisms、Genomics and Phylogenetic Studies

utilize one chemoreceptor per pathway, whereas the fourth pathway, which is the main system controlling chemotaxis, utilizes the other 23 chemoreceptors. Our results show that while only a very few amino acid positions in receptors, kinases, and adaptors determine their pathway specificity, assigning receptors to pathways computationally is possible. This requires substantial knowledge about interacting partners on a molecular level and focusing comparative sequence analysis on the pathway-specific regions. This general principle should be applicable to resolving many other receptor-pathway interactions.