A COI 1 gene in wheat contributes to the early defence response against wheat powdery mildew

作者：Xiaoying Liu, Junya Wang, Baoli Fan, Yuntao Shang, Yifan Sun, Chen Dang, Chaojie Xie, Zhenying Wang, Yongkang Peng · 发表于：Journal of Phytopathology · 年份：2017 · DOI：10.1111/jph.12667 · 被引用次数：15 · 研究领域：Insect-Plant Interactions and Control、Plant Parasitism and Resistance、Plant Virus Research Studies

Abstract The hormone jasmonate ( JA ) signalling pathway is involved in diverse developmental processes in plants. COI 1 is a key regulator in JA pathway. Although COI 1 homologous proteins have been identified in a variety of crops, their regulatory mechanisms in wheat defence responses remain unknown. Here, we cloned COI 1 from disease resistant wheat Brock, designated Ta COI 1 . Ta COI 1 cDNA was 1988‐bp long, with an ORF encoding 593 deduced amino acids. The phylogenetic tree showed that Ta COI 1 was clustered in the same clade of monocotyledons. The predicted protein possesses an F‐box domain, several LRR s and a conserved AMN 1 domain. Its transcription was induced in the compatible and incompatible wheat by Blumeria graminis f. sp. tritici ( Bgt ) fungus, with differences observed in response time. Expression pattern analysis in the susceptible wheat variety Jing411 revealed that Ta COI 1 was up‐regulated after Bgt inoculation at 12‐hour postinoculation (hpi). While, in Brock its transcription was rapidly induced and reached a first peak at 2 hpi. These results suggest that Ta COI 1 may be involved in the early response to powdery mildew. The expression of Ta COI 1 was decreased by virus‐induced gene silencing. The rate of successful penetration by Bgt was higher in all Ta COI 1 ‐silenced Brock, while the percentage of macrocephalic appressoria and resistance reactions was lower than control. These results suggest that silencing of the Ta COI 1 gene increased Brock sus...