Analysis of Mammalian O-Glycopeptides—We Have Made a Good Start, but There is a Long Way to Go

作者：Zsuzsanna Darula, Katalin F. Medzihradszky · 发表于：Molecular & Cellular Proteomics · 年份：2017 · DOI：10.1074/mcp.mr117.000126 · 被引用次数：103 · 研究领域：Glycosylation and Glycoproteins Research、Carbohydrate Chemistry and Synthesis、RNA and protein synthesis mechanisms

Glycosylation is perhaps the most common post-translational modification. Recently there has been growing interest in cataloging the glycan structures, glycoproteins, and specific sites modified and deciphering the biological functions of glycosylation. Although the results are piling up for N-glycosylation, O-glycosylation is seriously trailing behind. In our review we reiterate the difficulties researchers have to overcome in order to characterize O-glycosylation. We describe how an ingenious cell engineering method delivered exciting results, and what could we gain from "wild-type" samples. Although we refer to the biological role(s) of O-glycosylation, we do not provide a complete inventory on this topic.