scDual-Seq: mapping the gene regulatory program of Salmonella infection by host and pathogen single-cell RNA-sequencing

作者：Gal Avital, Roi Avraham, Amy C. Fan, Tamar Hashimshony, Deborah T. Hung, Itai Yanai · 发表于：Genome biology · 年份：2017 · 被引用次数：120 · 研究领域：Single-cell and spatial transcriptomics、RNA Research and Splicing、interferon and immune responses

The interaction between a pathogen and a host is a highly dynamic process in which both agents activate complex programs. Here, we introduce a single-cell RNA-sequencing method, scDual-Seq, that simultaneously captures both host and pathogen transcriptomes. We use it to study the process of infection of individual mouse macrophages with the intracellular pathogen Salmonella typhimurium. Among the infected macrophages, we find three subpopulations and we show evidence for a linear progression through these subpopulations, supporting a model in which these three states correspond to consecutive stages of infection.