The M6A methyltransferase METTL3: acting as a tumor suppressor in renal cell carcinoma

作者：Xiao Li, Jingyuan Tang, Wen R. H. Huang, Feng Wang, Pu Li, Chao Qin, Zhiqiang Qin, Qing Zou, Jifu Wei, Lixin Hua, Haiwei Yang, Zengjun Wang · 发表于：Oncotarget · 年份：2017 · DOI：10.18632/oncotarget.21726 · 被引用次数：236 · 研究领域：RNA modifications and cancer、Cancer-related gene regulation、Epigenetics and DNA Methylation

// Xiao Li 1, 2, * , Jingyuan Tang 1, 3, * , Wen Huang 4, * , Feng Wang 1 , Pu Li 1 , Chao Qin 1 , Zhiqiang Qin 1 , Qing Zou 2 , Jifu Wei 4 , Lixin Hua 1 , Haiwei Yang 1 and Zengjun Wang 1 1 State Key Laboratory of Reproductive Medicine, Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China 2 Department of Urology, Affiliated Cancer Hospital of Jiangsu Province of Nanjing Medical University, Nanjing 210009, China 3 Department of Urology, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing 210029,China 4 Research Division of Clinical Pharmacology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China * These authors have contributed equally to this work Correspondence to: Haiwei Yang, email: haiweiyang@njmu.edu.cn Jifu Wei, email: weijifu@hotmail.com Lixin Hua, email: hlx_urology@126.com Keywords: methyltransferase; METTL3; renal cell carcinoma Received: February 14, 2017 Accepted: July 25, 2017 Published: October 10, 2017 ABSTRACT We aimed to study the role of METTL3 in renal cell carcinoma (RCC) carcinogenesis and development. Immunohistochemistry was performed in clinical tissue microarray. Expression level of METTL3 in RCC tissues and cell lines was evaluated by quantitative real-time PCR (qRT-PCR) and western blot. Then, the effects of METTL3 on proliferation, migration, invasion and cell cycle were studied in RCC cells. Additionally, in vivo study was carried ...