Comparative Proteomic Analysis of Susceptible and Resistant Rice Plants during Early Infestation by Small Brown Planthopper

作者：Yan Dong, Xianping Fang, Yong Yang, Gang‐Ping Xue, Xian Chen, Weilin Zhang, Xüming Wang, Chulang Yu, Jie Zhou, Qiong Mei, Fang Wang, Chengqi Yan, Jianping Chen · 发表于：Frontiers in Plant Science · 年份：2017 · DOI：10.3389/fpls.2017.01744 · 被引用次数：31 · 研究领域：Plant Parasitism and Resistance、Insect-Plant Interactions and Control、Allelopathy and phytotoxic interactions

The small brown planthopper (Laodelphax striatellus Fallén, Homoptera, Delphacidae - SBPH) is one of the major destructive pests of rice (Oryza sativa L.). Understanding on how rice responds to SBPH infestation will contribute to developing strategies for SBPH control. However, the response of rice plant to SBPH is poorly understood. In this study, two contrasting rice genotypes, Pf9279-4 (SBPH-resistant) and 02428 (SBPH-susceptible), were used for comparative analysis of protein profiles in the leaf sheath of rice plants in responses to SBPH infestation. One hundred and thirty-two protein spots that were differentially expressed between the resistant and susceptible rice lines were identified with significant intensity differences (≥2-fold, P < 0.05) at 0 h, 6 h, and 12 h after SBPH infestation. Protein expression profile analysis in the leaf sheath of SBPH-resistant and SBPH-susceptible rice lines after SBPH infestation showed that proteins induced by SBPH feeding were involved mainly in stress response, photosynthesis, protein metabolic process, carbohydrate metabolic process, energy metabolism, cell wall-related proteins, amino acid metabolism and transcriptional regulation. Gene expression analysis of 24 differentially expressed proteins (DEPs) showed that more than 50% DEPs were positively correlated with their mRNA levels. Analysis of some physiological indexes mainly involved in the removal of oxygen reactive species showed that the levels of superoxide dismutase (SOD...