Genome-wide identification of leaf abscission associated microRNAs in sugarcane (Saccharum officinarum L.)

作者：Ming Li, Zhaoxu Liang, Shanshan He, Yuan Zeng, Jing Yan, Weikuan Fang, Kai-Chao Wu, Guanyu Wang, Xia Ning, Lunwang Wang, Song Li, Hongwei Tan, Fang Tan · 发表于：BMC Genomics · 年份：2017 · DOI：10.1186/s12864-017-4053-3 · 被引用次数：37 · 研究领域：Plant Molecular Biology Research、Plant Gene Expression Analysis、Polysaccharides and Plant Cell Walls

BACKGROUND: Sugarcane (Saccharum officinarum L.) is an economically important crop, mainly due to the production of sugar and biofuel (Azevedo RA, Carvalho RF, Cia MC, & Gratão PL, Trop Plant Biol 4:42-51, 2011). Grown mainly in tropical and subtropical countries, sugarcane is a highly polyploid plant with up to ten copies of each chromosome, which increases the difficulties of genome assembly and genetic, physiologic and biochemical analyses. The increasing demands of sugar and the increasing cost of sugarcane harvest require sugarcane varieties which can shed their leaves during the maturity time, so it is important to study the mechanism of leaf abscission in sugarcane. RESULTS: To improve the understanding of miRNA roles in sugarcane leaf abscission, we reported the genome-wide characterization of miRNAs and their putative targets in sugarcane using deep sequencing for six small RNA libraries. In total, 93 conserved miRNAs and 454 novel miRNAs were identified in sugarcane using previously reported transcriptome as reference. Among them, 25 up-regulated and 13 down-regulated miRNAs were identified in leaf abscission sugarcane plants (LASP) compared to leaf packaging sugarcane plants (LPSP). Target prediction revealed several miRNA-mRNA modules including miR156-SPL, miR319-TPR2, miR396-GRF and miR408-LAC3 might be involved in the sugarcane leaf abscission. KEGG pathway enrichment analysis showed differentially expressed miRNAs may regulate pathways like "plant hormone signa...