Mechanistic insight into the suppression of microglial ROS production by voltage-gated proton channels (VSOP/Hv1)

作者：Takafumi Kawai, Shoki Tatsumi, Shinji Kihara, Kenji Sakimura, Yasushi Okamura · 发表于：Channels · 年份：2017 · DOI：10.1080/19336950.2017.1385684 · 被引用次数：25 · 研究领域：Neuroinflammation and Neurodegeneration Mechanisms、Neuroscience and Neuropharmacology Research、Adenosine and Purinergic Signaling

Voltage-gated proton channels (VSOP/Hv1) reportedly promote reactive oxygen species (ROS) production in several immune cell types. However, we recently reported that primary microglia from VSOP/Hv1-deficient mice show higher ROS production than those from WT mice. Microglia may show a distinct activation status between WT and VSOP/Hv1-deficient cells, leading to a distinct level of ROS production between them. This is unlikely, however, because ROS production in VSOP/Hv1-deficient microglia remained higher than in WT microglia when the cells were exposed to LPS. Further, this increase in ROS production in VSOP/Hv1-deficient cells was not observed in macrophages, which suggests microglia have a unique mechanism of VSOP/Hv1-dependent ROS regulation. The mechanism underlying this unconventional ROS regulation by VSOP/Hv1 in microglia is discussed.