Porous Se@SiO<sub>2</sub> nanospheres treated paraquat-induced acute lung injury by resisting oxidative stress

作者：Yong Zhu, Guoying Deng, Anqi Ji, Jiayi Yao, Xiaoxiao Meng, Jinfeng Wang, Qian Wang, Qiugen Wang, Ruilan Wang · 发表于：International Journal of Nanomedicine · 年份：2017 · DOI：10.2147/ijn.s143192 · 被引用次数：50 · 研究领域：Paraquat toxicity studies and treatments、Heme Oxygenase-1 and Carbon Monoxide、Selenium in Biological Systems

Abstract: Acute paraquat (PQ) poisoning is one of the most common forms of pesticide poisoning. Oxidative stress and inflammation are thought to be important mechanisms in PQ-induced acute lung injury (ALI). Selenium (Se) can scavenge intracellular free radicals directly or indirectly. In this study, we investigated whether porous [email protected] 2 nanospheres could alleviate oxidative stress and inflammation in PQ-induced ALI. Male Sprague Dawley rats and RLE-6TN cells were used in this study. Rats were categorized into 3 groups: control (n=6), PQ (n=18), and PQ + [email protected] 2 (n=18). The PQ and PQ + [email protected] 2 groups were randomly and evenly divided into 3 sub-groups according to different time points (24, 48 and 72 h) after PQ treatment. Porous [email protected] 2 nanospheres 1 mg/kg (in the PQ + [email protected] 2 group) were administered via intraperitoneal injection every 24 h. Expression levels of reduced glutathione, malondialdehyde, superoxide dismutase, reactive oxygen species (ROS), nuclear factor-κB (NF-κB), phosphorylated NF-κB (p-NF-κB), tumor necrosis factor-α and interleukin-1β were detected, and a histological analysis of rat lung tissues was performed. The results showed that the levels of ROS, malondialdehyde, NF-κB, p-NF-κB, tumor necrosis factor-α and interleukin-1β were markedly increased after PQ treatment. Glutathione and superoxide dismutase levels were reduced. However, treatment with porous [email protected] 2 nanospheres markedly...