Msn2/4 regulate expression of glycolytic enzymes and control transition from quiescence to growth

作者：Zheng Kuang, Sudarshan Pinglay, Hongkai Ji, Jef D. Boeke · 发表于：eLife · 年份：2017 · DOI：10.7554/elife.29938 · 被引用次数：76 · 研究领域：Ubiquitin and proteasome pathways、RNA modifications and cancer、Genomics and Chromatin Dynamics

Nutrient availability and stresses impact a cell's decision to enter a growth state or a quiescent state. Acetyl-CoA stimulates cell growth under nutrient-limiting conditions, but how cells generate acetyl-CoA under starvation stress is less understood. Here, we show that general stress response factors, Msn2 and Msn4, function as master transcriptional regulators of yeast glycolysis via directly binding and activating genes encoding glycolytic enzymes. Yeast cells lacking Msn2 and Msn4 exhibit prevalent repression of glycolytic genes and a significant delay of acetyl-CoA accumulation and reentry into growth from quiescence. Thus Msn2/4 exhibit a dual role in activating carbohydrate metabolism genes and stress response genes. These results suggest a possible mechanism by which starvation-induced stress response factors may prime quiescent cells to reenter growth through glycolysis when nutrients are limited.