An enzymatic strategy to asymmetrically branched N-glycans

作者：Angie D. Calderon, Jun Zhou, Wanyi Guan, Zhigang Wu, Yuxi Guo, Jing Bai, Qing Li, Peng George Wang, Junqiang Fang, Lei Li · 发表于：Organic & Biomolecular Chemistry · 年份：2017 · DOI：10.1039/c7ob01765k · 被引用次数：26 · 研究领域：Carbohydrate Chemistry and Synthesis、Glycosylation and Glycoproteins Research、Enzyme Production and Characterization

An enzymatic strategy was developed to generate asymmetrically branched N-glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ β-galactosidase was employed to selectively trim symmetrically branched N-glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension.