Structural Characterization of Intact Glycoconjugates by Tandem Mass Spectrometry Using Electron-Induced Dissociation

作者：Yum‐Shing Wong, Xiangfeng Chen, Ri Wu, Y.-L. Winnie Hung, T.‐W. Dominic Chan · 发表于：Analytical Chemistry · 年份：2017 · DOI：10.1021/acs.analchem.7b03128 · 被引用次数：24 · 研究领域：Mass Spectrometry Techniques and Applications、Glycosylation and Glycoproteins Research、Analytical Chemistry and Chromatography

Characterizing the structures of glycoconjungates is important because of glycan heterogeneity and structural complexity of aglycon. The presence of relatively weak glycosidic linkages leads to preferential cleavages that limit the acquisition of structural information under typical mass spectrometry dissociation conditions, such as collision-induced dissociation (CID) and infrared multiphoton dissociation. In this paper, we explored the dissociation behaviors of different members of glycoconjugates, including glycopeptides, glycoalkaloids, and glycolipids, under electron-induced dissociation (EID) conditions. Using CID spectra as references, we found that EID is not only a complementary method to CID, but also a method that can generate extensive fragment ions for the structural characterization of all intact glycoconjugates studied. Furthermore, isomeric ganglioside species can be differentiated, and the double bond location in the ceramide moiety of the gangliosides can be identified through the MS 3 approach involving sequential CID and EID processes.