Sugar metabolism of the first thermophilic planctomycete Thermogutta terrifontis: comparative genomic and transcriptomic approaches

作者：Alexander G. Elcheninov, Peter Menzel, Soley Gudbergsdottir, Alexeï Slesarev, Vitaly V. Kadnikov, Anders Krogh, E. A. Bonch-Osmolovskaya, X. -S. Peng, Ilya V. Kublanov · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2017 · DOI：10.1101/179903 · 被引用次数：2 · 研究领域：Enzyme Production and Characterization、Polysaccharides and Plant Cell Walls、Fungal Biology and Applications

Abstract Xanthan gum, a complex polysaccharide comprising glucose, mannose and glucuronic acid residues, is involved in numerous biotechnological applications in cosmetics, agriculture, pharmaceuticals, food and petroleum industries. Additionally, its oligosaccharides were shown to possess antimicrobial, antioxidant and few other properties. Yet, despite its extensive usage, little is known about xanthan gum degradation pathways and mechanisms. Thermogutta terrifontis R1 was described as the first thermophilic representative of the Planctomycetes phylum. As other cultivated planctomycetes, it grows well on various carbohydrates including oligo- and polysaccharides, however, its capability of anaerobic growth with or without electron acceptors was a novel finding among the representatives of this phylum. The aim of this work is to examine T. terrifontis catabolic pathways with a special focus on the xanthan gum degradation pathway using genomic and transriptomic sequencing. Genomic analysis revealed more than a hundred glycosidases, polysaccharide lyases and other CAZymes, involved in oligo- and polysaccharide degradation by T. terrifontis , proteins of central carbohydrate metabolism and aerobic and anaerobic respiration. Furthermore, the combination of genomic and transcriptomic approaches revealed a putative novel xanthan gum degradation pathway involving unusual catalytic steps and enzymes: novel glycosidase(s) of DUF1080 family, hydrolyzing xanthan gum beta-glucosidic bac...