Thiol–ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles

作者：Yuan Liu, Weijia Hou, Hao Sun, Cheng Cui, Liqin Zhang, Ying Jiang, Yong‐Xiang Wu, Yanyue Wang, Juan Li, Brent S. Sumerlin, Qiaoling Liu, Weihong Tan · 发表于：Chemical Science · 年份：2017 · DOI：10.1039/c7sc01447c · 被引用次数：117 · 研究领域：Click Chemistry and Applications、Monoclonal and Polyclonal Antibodies Research、Advanced biosensing and bioanalysis techniques

Bioconjugation based on crosslinking primary amines to carboxylic acid groups has found broad applications in protein modification, drug development, and nanomaterial functionalization. However, proteins, which are made up of amino acids, typically give nonselective bioconjugation when using primary amine-based crosslinking. In order to control protein orientation and activity after conjugation, selective bioconjugation is desirable. We herein report an efficient and cysteine-selective thiol-ene click reaction-based bioconjugation strategy using colloidal nanoparticles. The resulting thiol-ene based aptamer and enzyme nanoconjugates demonstrated excellent target binding ability and enzymatic activity, respectively. Thus, thiol-ene click chemistry can provide a stable and robust crosslinker in a biocompatible manner for bioconjugation of any thiol-containing biomolecule with nanomaterials. This will open more opportunities for applications of thiol-ene reactions and functional colloidal nanoparticles in chemical biology.