Metabolic engineering of yeast for fermentative production of flavonoids

作者：Angelica Rodriguez, Tomas Strucko, Steen Gustav Stahlhut, Mette Kristensen, Daniel Killerup Svenssen, Jochen Förster, JENS KVIST NIELSEN, Irina Borodina · 发表于：Bioresource Technology · 年份：2017 · DOI：10.1016/j.biortech.2017.06.043 · 被引用次数：190 · 研究领域：Plant biochemistry and biosynthesis、Microbial Metabolic Engineering and Bioproduction、Plant Gene Expression Analysis

Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 ± 2.66 mg L−1 and quercetin 20.38 ± 2.57 mg L−1 exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.