miR-26b Promotes 3T3-L1 Adipocyte Differentiation Through Targeting PTEN

作者：Guilin Li, Chunyou Ning, Yao Ma, Long Jin, Qianzi Tang, Xuewei Li, Mingzhou Li, Haifeng Liu · 发表于：DNA and Cell Biology · 年份：2017 · DOI：10.1089/dna.2017.3712 · 被引用次数：35 · 研究领域：MicroRNA in disease regulation、Cancer-related molecular mechanisms research、RNA Research and Splicing

microRNAs (miRNAs) play important roles in adipogenesis that is closely linked to obesity and energy homeostasis. Thus far, only a few miRNAs have been identified to regulate adipocyte development, arousing interest in the detailed function of miRNAs during adipogenesis. In this study, we found that the miR-26b expression showed an increasing trend during 3T3-L1 cells differentiation. To investigate the role of miR-26b in adipogenesis, the synthetic miR-26b agomirs and antagomirs were used to perform overexpression and knockdown experiment, respectively. Our data revealed that overexpression of miR-26b significantly accelerated the mRNA expression of the adipogenic markers, peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), CCAAT/enhancer binding protein alpha (C/EBPα), and lipoprotein lipase, and the protein level of PPARγ and FAS. miR-26b overexpression also resulted in a significant increase in lipid accumulation. In contrast, inhibition of miR-26b expression decreased differentiation of 3T3-L1 cells. By target gene prediction and luciferase reporter assay, we demonstrated that miR-26b may directly bind to the 3' UTR of phosphatase and tensin homolog (PTEN). Taken together, these results demonstrate that miR-26b might participate in regulating adipogenic differentiation in 3T3-L1 cells by inhibiting the PTEN expression, further highlighting the importance of miRNA in adipogenesis.