A subcellular map of the human proteome

作者：Peter J. Thul, Lovisa Åkesson, Mikaela Wiking, Diana Mahdessian, Aikaterini Geladaki, Hammou Ait Blal, Tove Alm, Anna Asplund, Lars E. Björk, Lisa M. Breckels, Anna Bäckström, Frida Danielsson, Linn Fagerberg, Jenny Fall, Laurent Gatto, Christian Gnann, Sophia Hober, Martin Hjelmare, Fredric Johansson, Sunjae Lee, Cecilia Lindskog, Jan Mulder, Claire M. Mulvey, Peter Nilsson, Per Oksvold, Johan Rockberg, Rutger Schutten, Jochen M. Schwenk, Åsa Sivertsson, Evelina Sjöstedt, Marie Skogs, Charlotte Stadler, Devin P. Sullivan, Hanna Tegel, Casper F. Winsnes, Cheng Zhang, Martin Zwahlen, Adil Mardinoğlu, Fredrik Pontén, Kalle von Feilitzen, Kathryn Susan Lilley, Mathias Uhlén, Emma K. Lundberg · 发表于：Science · 年份：2017 · DOI：10.1126/science.aal3321 · 被引用次数：3049 · 研究领域：Advanced Proteomics Techniques and Applications、Bioinformatics and Genomic Networks、Cell Image Analysis Techniques

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.