Level‐specific amputations and resulting regenerative outcomes in the mouse distal phalanx

作者：Connie S. Chamberlain, Justin J. Jeffery, Ellen M. Leiferman, Tuğrul Yıldırım, Xin Sun, Geoffrey S. Baer, William L. Murphy, Ray Vanderby · 发表于：Wound Repair and Regeneration · 年份：2017 · DOI：10.1111/wrr.12544 · 被引用次数：30 · 研究领域：Reconstructive Surgery and Microvascular Techniques、Wound Healing and Treatments、Surgical Sutures and Adhesives

Mouse digit tip regeneration involves an intricate coordinated regrowth of the terminal phalanx, nail, dermis and epidermis. During this time, regenerating digits undergo wound healing, blastema formation, and differentiation. However, the regenerative response of the digit is dependent on the level of the amputation. Amputation of <30% of the distal phalanx (P3), with part of the base nail remaining, results in extensive digit regeneration. In contrast, >60% P3 removal results in no regeneration. This level-dependent regenerative ability of the mouse digit provides a comparative model between regeneration and non-regeneration that may enable identification of specific factors critical to regeneration. Although the ability to create regenerating and non-regenerating conditions has been well established, the regenerative response between these regions ("intermediate" zone) has received less scrutiny, and may add insight to the regenerative processes, including the degree of histolysis, and the level of blastema formation. The objective of this study is then to compare the regeneration capacity between amputation levels within the regenerating (<30%), intermediate (40-59%), and non-regenerating (>60%) regions. Results indicated that regenerative and intermediate amputations led to significant histolysis and blastema formation of the distal phalanx 14 days post-amputation. Unlike the regenerating digits, intermediate amputations led to incomplete regeneration whereby regrowth of...