An electrochemiluminescence assay for sensitive detection of methyltransferase activity in different cancer cells by hybridization chain reaction coupled with a G-quadruplex/hemin DNAzyme biosensing strategy

作者：Hui Zhang, Zhihui Guo, Huilei Dong, Hongfei Chen, Chenxin Cai · 发表于：The Analyst · 年份：2017 · DOI：10.1039/c7an00486a · 被引用次数：25 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、RNA modifications and cancer

in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells. This ECL assay provides a promising platform for profiling of the mutational cells of tumors and shows a great potential for application to DNA methylation-related clinical diagnostics.