CXCR5-Overexpressing Mesenchymal Stromal Cells Exhibit Enhanced Homing and Can Decrease Contact Hypersensitivity

作者：Xiaoran Zhang, Weijun Huang, Xiaoyong Chen, Yufan Lian, Jiancheng Wang, Chuang Cai, Li Huang, Tao Wang, Jie Ren, Andy Peng Xiang · 发表于：Molecular Therapy · 年份：2017 · DOI：10.1016/j.ymthe.2017.04.004 · 被引用次数：63 · 研究领域：Mesenchymal stem cell research、Wound Healing and Treatments、Extracellular vesicles in disease

Mesenchymal stromal cells (MSCs) can modulate inflammation and contribute to tissue regeneration and, thus, have emerged as a promising option for cell-based therapy. However, the ability of MSCs to migrate to injured tissues still needs to be improved. In this study, we investigated whether genetically engineered MSCs could exhibit increased migratory properties and improved therapeutic efficacy. Using a mouse model of contact hypersensitivity (CHS), chemokine gene expression screening revealed that CXCL13 changed most significantly in injured tissue. Unfortunately, MSCs hardly express the corresponding receptor, CXCR5. Thus, CXCR5-overexpressing MSCs (MSC CXCR5 ) were generated that retained their abilities of proliferation, differentiation, and immunomodulation. Furthermore, MSC CXCR5 showed significantly increased migrating ability toward CXCL13. Importantly, systemic infusion of MSC CXCR5 dramatically suppressed CHS in mice, as evidenced by decreased levels of inflammatory cell infiltration and pro-inflammatory cytokine production. Numerous MSC CXCR5 migrated into inflamed ears, localized with T cells, inhibited T cell proliferation, promoted T cell apoptosis, and suppressed the production of T cell-derived pro-inflammatory factors. Collectively, these findings demonstrate that CXCR5 overexpression increases the ability of MSCs to respond to migratory stimuli and highly intensifies their immunomodulatory effects in vivo. This strategy for enhancing targeted stem/progenit...