Nanopore Logic Operation with DNA to RNA Transcription in a Droplet System

作者：Masayuki Ohara, Masahiro Takinoue, Ryuji Kawano · 发表于：ACS Synthetic Biology · 年份：2017 · DOI：10.1021/acssynbio.7b00101 · 被引用次数：47 · 研究领域：Nanopore and Nanochannel Transport Studies、Advanced biosensing and bioanalysis techniques、Quantum-Dot Cellular Automata

This paper describes an AND logic operation with amplification and transcription from DNA to RNA, using T7 RNA polymerase. All four operations, (0 0) to (1 1), with an enzyme reaction can be performed simultaneously, using four-droplet devices that are directly connected to a patch-clamp amplifier. The output RNA molecule is detected using a biological nanopore with single-molecule translocation. Channel current recordings can be obtained using the enzyme solution. The integration of DNA logic gates into electrochemical devices is necessary to obtain output information in a human-recognizable form. Our method will be useful for rapid and confined DNA computing applications, including the development of programmable diagnostic devices.