MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells

作者：Chunyu Bai, Yuhua Gao, Xiangchen Li, Kunfu Wang, Hui Xiong, Zhiqiang Shan, Ping Zhang, Wenjie Wang, Weijun Guan, Yuehui Ma · 发表于：Journal of Tissue Engineering and Regenerative Medicine · 年份：2017 · DOI：10.1002/term.2259 · 被引用次数：38 · 研究领域：Pancreatic function and diabetes、MicroRNA in disease regulation、Mesenchymal stem cell research

MicroRNAs regulate insulin secretion, pancreatic development and beta cell differentiation. However, the function of microRNAs in the formation of insulin-producing cells (IPCs) from adult stem cells is poorly understood. We examine the microRNA expression profile in nestin-positive umbilical cord-derived mesenchymal stem cells (N-UCMSCs) and nestin-positive pancreatic mesenchymal stem cells using a deep sequencing approach. We also selected specific microRNAs for overexpression in N-UCMSCs and found that miR-375 and miR-26a induced IPCs differentiation from N-UCMSCs by downregulating target genes including mtpn, sox6, bhlhe22 and ccnd1. Small interfering RNAs were also used to knock down these genes in N-UCMSCs to induce the formation of IPCs. These results suggest that endogenous microRNAs involved in the formation of IPCs from adult stem cells show promise for advancing the development of an effective cell transplant therapy for diabetes. Copyright © 2017 John Wiley & Sons, Ltd.