Isolation and characterization of microsatellite loci for Smilax sieboldii (Smilacaceae)

作者：Yalu Ru, Ruijing Cheng, Jing Shang, Yunpeng Zhao, Pan Li, Chengxin Fu · 发表于：Applications in Plant Sciences · 年份：2017 · DOI：10.3732/apps.1700001 · 被引用次数：4 · 研究领域：Phytochemical Studies and Bioactivities、Genetic diversity and population structure、Plant Pathogens and Fungal Diseases

Premise of the study: Polymorphic microsatellite markers were developed for Smilax sieboldii (Smilacaceae), a member of the S. hispida group with a biogeographic disjunction between eastern Asia and North America, to study the phylogeography and incipient speciation of this species and its close relatives. Methods and Results: Transcriptome sequencing produced 47,628 unigenes. Seventeen loci were developed from 122 randomly selected primer pairs. Polymorphism and genetic variation were evaluated for 68 accessions representing five populations of S. sieboldii . The number of alleles per locus ranged from four to 18; the expected heterozygosity ranged from 0.59 to 0.92. Twelve loci were successfully amplified in five related species: S. scobinicaulis, S. californica, S. hispida, S. moranensis , and S. jalapensis . Conclusions: These novel expressed sequence tag–derived microsatellite markers will facilitate further population genetic research of S. sieboldii and its close allies of the S. hispida group.