Botryococcus braunii strains compared for biomass productivity, hydrocarbon and carbohydrate content

作者：Joao Gouveia, Jesús Ruiz, L.A.M. van den Broek, Thamara Hesselink, Sander Peters, Dorinde M.M. Kleinegris, Alison G. Smith, Douwe van der Veen, María J. Barbosa, René H. Wijffels · 发表于：Journal of Biotechnology · 年份：2017 · DOI：10.1016/j.jbiotec.2017.03.008 · 被引用次数：72 · 研究领域：Biofuel production and bioconversion、Algal biology and biofuel production、Microbial Metabolic Engineering and Bioproduction

Botryococcus braunii can produce both long-chain hydrocarbons as well as carbohydrates in large quantities, and is therefore a promising industrial organism for the production of biopolymer building blocks. Many studies describe the use of different strains of Botryococcus braunii but differences in handling and cultivation conditions make the comparison between strains difficult. In this study, 16 B. braunii strains obtained from six culture collections were compared for their biomass productivity and hydrocarbon and carbohydrate content. Biomass productivity was highest for AC768 strain with 1.8 g L−1 day−1, while hydrocarbon production ranged from none to up to 42% per gram biomass dry weight, with Showa showing the highest hydrocarbon content followed by AC761. The total carbohydrate content varied from 20% to 76% per gram of the biomass dry weight, with CCALA777 as the highest producer. Glucose and galactose are the main monosaccharides in most strains and fucose content reached 463 mg L−1 in CCALA778.