Pseudogene PHBP1 promotes esophageal squamous cell carcinoma proliferation by increasing its cognate gene PHB expression

作者：Feiyue Feng, Bin Qiu, Ruochuan Zang, Peng Song, Shugeng Gao · 发表于：Oncotarget · 年份：2017 · DOI：10.18632/oncotarget.16196 · 被引用次数：21 · 研究领域：Cancer-related molecular mechanisms research、RNA modifications and cancer、RNA Research and Splicing

// Feiyue Feng 1 , Bin Qiu 1 , Ruochuan Zang 1 , Peng Song 1 , Shugeng Gao 1 1 Department of Thoracic Surgery, National Cancer Center, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China Correspondence to: Feiyue Feng, email: feiyuefeng_cn@aliyun.com Shugeng Gao, email: gaoshugeng@vip.sina.com Keywords: natural antisense transcript (NAT), long noncoding RNA (lncRNA), esophageal squamous cell carcinoma (ESCC), quantitative real-time PCR (qRT-PCR), SDS-polyacrylamide gel electrophoresis (SDS-PAGE) Received: January 02, 2017      Accepted: January 25, 2017      Published: March 16, 2017 ABSTRACT Natural antisense transcripts (NATs) as one of the most diverse classes of long noncoding RNAs (lncRNAs), have been demonstrated involved in fundamental biological processes in human. Here, we reported that human prohibitin gene pseudogene 1 ( PHBP1 ) was upregulated in ESCC, and increased PHBP1 expression in ESCC was associated with clinical advanced stage. Functional experiments showed that PHBP1 knockdown inhibited ESCC cells proliferation, colony formation and xenograft tumor growth in vitro and in vivo by causing cell-cycle arrest at the G1-G0 phase. Mechanisms analysis revealed that PHBP1 transcript as an antisense transcript of PHB is partially complementary to PHB mRNA and formed an RNA-RNA hybrid with PHB , consequently inducing an increase of PHB expression at both the mRN...