Tetrandrine, an agonist of aryl hydrocarbon receptor, reciprocally modulates the activities of STAT3 and STAT5 to suppress Th17 cell differentiation

作者：Xusheng Yuan, Yannong Dou, Xin Wu, Zhifeng Wei, Yue Dai · 发表于：Journal of Cellular and Molecular Medicine · 年份：2017 · DOI：10.1111/jcmm.13141 · 被引用次数：31 · 研究领域：Immunotherapy and Immune Responses、Cytokine Signaling Pathways and Interactions、T-cell and B-cell Immunology

Abstract Tetrandrine, a bisbenzylisoquinoline alkaloid constituent of the root of Stephania tetrandra S. Moore, was previously shown to suppress the differentiation of T helper 17 (Th17) cells and consequently ameliorate the collagen‐induced arthritis (CIA) in mice by activating the aryl hydrocarbon receptor (AhR), but its underlying mechanism is incompletely understood. Here, we investigated how tetrandrine suppressed Th17 cell differentiation through the AhR pathway. The naïve CD4+ T cells were stimulated with anti‐CD3/CD28 for 72 hrs in the presence or absence of tetrandrine under the Th17‐polarizing condition. Tetrandrine inhibited the phosphorylation of signal transducer and activator of transcription‐3 (STAT3) and boosted the phosphorylation of STAT5, while it did not alter the expression levels of phospho‐Janus kinase‐1 (p‐JAK1), p‐JAK2, p‐JAK3, and suppressor of cytokine signalling‐3 (SOCS3). The tetrandrine‐mediated inhibition of the Th17 cell differentiation could be diminished by the activator of STAT3 and the inhibitor of STAT5. Meanwhile, the effect of tetrandrine on the either STAT3 or STAT5 phosphorylation was almost completely reversed by the AhR antagonist CH223191 and the AhR knockdown. In CIA mice, tetrandrine decreased p‐STAT3 levels and increased p‐STAT5 levels, which could also be reversed by the AhR antagonist resveratrol administration. Furthermore, tetrandrine promoted the AhR binding to the STAT5, but not to the STAT3. The tetrandrine‐induced inhibit...