Differential somatic cell count—A novel method for routine mastitis screening in the frame of Dairy Herd Improvement testing programs

作者：Malin Damm, Claus Holm, Mette Blaabjerg, Morten Novak Bro, Daniel R. Schwarz · 发表于：Journal of Dairy Science · 年份：2017 · DOI：10.3168/jds.2016-12409 · 被引用次数：164 · 研究领域：Milk Quality and Mastitis in Dairy Cows、Microbial infections and disease research、Infant Nutrition and Health

Somatic cell count (SCC) is used as key indicator in mastitis screening programs typically applied in the frame of Dairy Herd Improvement (DHI) testing programs. However, mastitis is still causing tremendous economic losses to the dairy industry. Hence, new biomarkers are needed that can be used for enhanced management of mastitis on dairy farms. Besides the determination of SCC, differentiation of cells has been described to be beneficial for a more definite description of the actual udder health status of dairy cows. The aim of this study was to develop a new method for rapid and simultaneous determination of SCC and a new parameter, differential somatic cell count (DSCC), in individual cow milk samples using flow cytometry. The method is sought to be applied in central milk testing laboratories, so that existing DHI infrastructures can be used. The DSCC represents the combined proportion of polymorphonuclear leukocytes (PMN) and lymphocytes expressed in percentage. The proportion of macrophages can be calculated by subtracting DSCC from 100%. Our research revealed increasing proportions of PMN, but decreasing proportions of macrophages as SCC increased. However, lymphocytes occurred fairly constantly with low proportions across the entire SCC range. Hence, the DSCC parameter reflects the antidromic trend of PMN and macrophages. Fluorescence microscopy was used to evaluate the specificity of the new Foss DSCC method in terms of DSCC and a high correlation was found. Apart f...