Mixed Self-Assembled Aptamer and Newly Designed Zwitterionic Peptide as Antifouling Biosensing Interface for Electrochemical Detection of alpha-Fetoprotein

作者：Min Cui, Yu Wang, Mingxia Jiao, Silambarasan Jayachandran, Yumin Wu, Xiaojian Fan, Xiliang Luo · 发表于：ACS Sensors · 年份：2017 · DOI：10.1021/acssensors.7b00103 · 被引用次数：160 · 研究领域：Advanced biosensing and bioanalysis techniques、Molecular Junctions and Nanostructures、RNA Interference and Gene Delivery

A sensitive and low-fouling aptasensor for alpha-fetoprotein (AFP) was developed based on mixed self-assembled aptamers and newly designed zwitterionic peptides, where densely immobilized peptides formed an antifouling layer to resist nonspecific protein adsorption, and sparsely attached aptamers acted as the recognizing layer to achieve target binding. The obtained biosensing interface responded to the target AFP with a strikingly selective and sensitive manner, exhibited excellent protein-resistant performance even in complex human serum solution, and showed promising feasibility for the quantitative analysis of AFP in real human serum samples.