Immunochemical Studies on Blood Groups

作者：Peter Z. Allen, Elvin A. Kabat · 发表于：The Journal of Immunology · 年份：1959 · DOI：10.4049/jimmunol.82.4.340 · 被引用次数：103 · 研究领域：Blood groups and transfusion、Hyperglycemia and glycemic control in critically ill and hospitalized patients、Diabetes and associated disorders

Summary The nondialyzable residue of mild acid treated blood group A or B substance (P1 fractions) can give rise in human beings to precipitins specific for the A P1 and B P1 fractions respectively. A P1 fractions differ from untreated A substance in that they are able to stimulate the formation of A P1 specific precipitins in group A as well as group O individuals. Similarly B P1 fractions differ from untreated B substance since they may give rise to B P1 specific precipitins in group AB as well as group O subjects. Anti-B P1 precipitins are specific for groupings present in B substance which are exposed by mild acid hydrolysis since untreated B preparations show little or no reactivity with anti-B P1 sera but acquire this capacity following mild acid treatment. Hog A substances likewise show no capacity to remove anti-A P1 precipitins unless subjected to mild acid treatment. A single human A substance tested, however, was found to possess A P1 specificity prior to treatment. Quantitative oligosaccharide inhibition studies indicate that B P1 specificity is partially determined by terminal nonreducing α-galactosyl residues. These α-galactosyl determinants of B P1 specificity appear to be involved in a linkage different from that of α-galactosyl determinants of blood group B specificity since melibiose which is very effective in inhibiting B-anti-B precipitation, is a poor inhibitor of B P1 anti-B P1, being even less effective in this system than galactose. Thus the galactosyl...