Pilot study on peptide purity—synthetic human C-peptide

作者：R D Josephs, Ming Li, Di Song, Adeline Daireaux, Tiphaine Choteau, Norbert Stoppacher, Steven Westwood, Robert Wielgosz, Peng Xiao, Y Liu, Xiang Gao, C Zhang, T Zhang, Wu Mi, Caixiong Quan, Ting Huang, H Li, Jeremy E. Melanson, İlker Ün, Ahmet C. Gören, Milena Quaglia, Juanita A. Warren · 发表于：Metrologia · 年份：2017 · DOI：10.1088/0026-1394/54/1a/08011 · 被引用次数：8 · 研究领域：Metabolomics and Mass Spectrometry Studies、Enzyme Structure and Function、Mass Spectrometry Techniques and Applications

Under the auspices of the Protein Analysis Working Group (PAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P55.2, was coordinated by the Bureau International des Poids et Mesures (BIPM) and the Chinese National Institute of Metrology (NIM). Four Metrology Institutes or Designated Institutes and the BIPM participated. Participants were required to assign the mass fraction of human C-peptide (hCP) present as the main component in the comparison sample for CCQM-P55.2. The comparison samples were prepared from synthetic human hCP purchased from a commercial supplier and used as provided without further treatment or purification. hCP was selected to be representative of the performance of a laboratory's measurement capability for the purity assignment of short (up to 5 kDa), non-cross-linked synthetic peptides/proteins. It was anticipated to provide an analytical measurement challenge representative for the value-assignment of compounds of broadly similar structural characteristics. The majority of participants used a quantitative nuclear magnetic resonance spectroscopy (qNMR) corrected for peptide impurities. Other participants provided results obtained by peptide impurity corrected amino acid analysis (PICAA) or elemental analysis (PICCHN). It was decided to assign reference values based on the KCRVs of CCQM-K115 for both the hCP mass fraction and the mass fraction of the peptide related impurities as indispensable contributor regardless of ...