Successful Allogenic Bone Marrow Transplantation in Man: Chimerism, Induced Specific Tolerance and Possible Anti-Leukemic Effects

作者：Georges Mathé, Jeanne Amiel, L Schwarzenberg, A. R. Cattan, Maurice Schneider, Marco J. De Vries, M Tubiana, C. M. Lalanne, J L Binet, Martine Papiernik, GABRIEL SEMAN, Makoto Matsukura, A.M. Mery, V. Schwarzmann, A Flaisler · 发表于：Blood · 年份：1965 · DOI：10.1182/blood.v25.2.179.179 · 被引用次数：165 · 研究领域：Hematopoietic Stem Cell Transplantation、Virus-based gene therapy research、RNA Interference and Gene Delivery

Abstract A patient suffering from acute lymphoblastic leukemia in the third progressive phase, refractory to the majority of available treatments, was given total-body irradiation of 800 r. dosage, after administration of methyl-nitro-imidazolyl-mercaptopurine (300 mg. daily for 4 days). One week after irradiation, he received an infusion of a mixture of equal parts of bone marrow from six donors (mother, sister, father and three brothers), a total of 5.8 x 1010 nucleated cells. Myeloid restoration began 2 weeks after the infusion and continued to progress rapidly. The reality of the graft was demonstrated by study of the erythrocytic antigens. Nearly all the erythrocytes found in the blood after 6 months belonged to the phenotype of one of the male donors. Studies of chromosomes and leukocyte sex chromatin suggested that a small proportion of leukocytes were being produced by the cells of one of the female donors. The patient was found to produce beta 2A, beta 2M and gamma globulins of the Inv type of the donors, suggesting that lymphoid cells may also have been successfully grafted. A skin graft from the male donor whose marrow graft had apparently been accepted was still perfectly intact 7 months after grafting, whereas the grafts from other donors were rejected, indicating a specific tolerance to tissues from this donor. Previous studies of compatibility between the recipient and the various donors by a histocompatibility test described by us, or by means of similarities ...