MNS16A tandem repeat minisatellite of human telomerase gene: functional studies in colorectal, lung and prostate cancer

作者：Philipp Hofer, Cornelia Zöchmeister, Christian Behm, Stefanie Brezina, Andreas Baierl, Angelina Doriguzzi, Vanita Vanas, Klaus Holzmann, Hedwig Sutterlüty, Andrea Gsur · 发表于：Oncotarget · 年份：2017 · DOI：10.18632/oncotarget.15884 · 被引用次数：12 · 研究领域：Telomeres, Telomerase, and Senescence、DNA Repair Mechanisms、Chromosomal and Genetic Variations

// Philipp Hofer 1 , Cornelia Zöchmeister 1 , Christian Behm 1 , Stefanie Brezina 1 , Andreas Baierl 2 , Angelina Doriguzzi 1 , Vanita Vanas 1 , Klaus Holzmann 1 , Hedwig Sutterlüty-Fall 1 , Andrea Gsur 1 1 Medical University of Vienna, Institute of Cancer Research, A-1090 Vienna, Austria 2 University of Vienna, Department of Statistics and Operations Research, A-1010 Vienna, Austria Correspondence to: Andrea Gsur, email: andrea.gsur@meduniwien.ac.at Keywords: genetic variation, MNS16A, functional polymorphism, telomerase, TERT regulation Received: September 23, 2016 Accepted: February 21, 2017 Published: March 03, 2017 ABSTRACT MNS16A, a functional polymorphic tandem repeat minisatellite, is located in the promoter region of an antisense transcript of the human telomerase reverse transcriptase gene. MNS16A promoter activity depends on the variable number of tandem repeats (VNTR) presenting varying numbers of transcription factor binding sites for GATA binding protein 1. Although MNS16A has been investigated in multiple cancer epidemiology studies with incongruent findings, functional data of only two VNTRs (VNTR-243 and VNTR-302) were available thus far, linking the shorter VNTR to higher promoter activity. For the first time, we investigated promoter activity of all six VNTRs of MNS16A in cell lines of colorectal, lung and prostate cancer using Luciferase reporter assay. In all investigated cell lines shorter VNTRs showed higher promoter activity. While this anticipated ind...