Assessment of Magnaporthe grisea mating type by spore PCR

作者：Jin‐Rong Xu, John E. Hamer · 发表于：Fungal Genetics Reports · 年份：1995 · DOI：10.4148/1941-4765.1358 · 被引用次数：40 · 研究领域：Plant Pathogens and Fungal Diseases、Lichen and fungal ecology、Yeasts and Rust Fungi Studies

Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In Saccharomyces cerevisiae, PCR assay can be performed with yeast colonies (Huxley et al. TIG 1990 6:236). Here we describe a PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA.