Increased Circulating Exosomal miRNA-223 Is Associated with Acute Ischemic Stroke

作者：Yajing Chen, Yaying Song, Jun Huang, Meijie Qu, Yu Zhang, Jieli Geng, Zhijun Zhang, Jianrong Liu, Guo‐Yuan Yang · 发表于：Frontiers in Neurology · 年份：2017 · DOI：10.3389/fneur.2017.00057 · 被引用次数：205 · 研究领域：Extracellular vesicles in disease、MicroRNA in disease regulation、Circular RNAs in diseases

Recent studies have demonstrated that exosomal microRNAs (miRNAs) are novel biomarkers and therapeutic targets for various diseases including vascular disease. However, specific exosomal miRNAs expression in stroke patients has not been reported yet. Here we explored whether circulating exosomal miRNAs can serve as potential biomarkers for the diagnosis of acute ischemic stroke and discussed the potential for clinical application. Blood samples were collected from acute ischemic stroke patients within the first 72 hours (n=50). Circulating exosomes were exacted by Exoquick exosome isolation kit and characterized by transmission electron microscopy (TEM). Western Blot was performed to assess the expression of exosomal protein makers. Exosomal microRNA-223 (miR-223) was detected by RT-PCR assay. The relationship between the expression levels of miR-223 and national institutes of health stroke scale (NIHSS) scores, brain infarct volume and neurological outcomes were analyzed. Circulating exosomes were isolated and the size of vesicles ranged between 30 and 100 nm. The identification of exosomes was further confirmed by the detection of specific exosomal protein markers CD9, CD63 and Tsg101. Exosomal miR-223 in acute ischemic stroke patients was significantly up-regulated compared to control group (p<0.001). Exosomal miR-223 level was positively correlated with NIHSS scores (r=0.31, p=0.03). Exosomal miR-223 expression in stroke patients with poor outcomes was higher than those w...