Key comparison study on peptide purity—synthetic human C-peptide

作者：R D Josephs, Ming Li, Di Song, Steven Westwood, Norbert Stoppacher, Adeline Daireaux, Tiphaine Choteau, Robert Wielgosz, Peng Xiao, Y Liu, Xiang Gao, C Zhang, T Zhang, Wu Mi, Caixiong Quan, Ting Huang, H Li, R.B. Flatschart, R Borges Oliveira, Jeremy E. Melanson, Rüdiger Ohlendorf, André Henrion, T Kinumi, Lilian L. L. Wong, Q Liu, M Oztug Senal, Bilgin Vatansever, İlker Ün, Ahmet C. Gören, Müslüm Akgöz, Milena Quaglia, Juanita A. Warren · 发表于：Metrologia · 年份：2017 · DOI：10.1088/0026-1394/54/1a/08007 · 被引用次数：19 · 研究领域：Computational Drug Discovery Methods、Mass Spectrometry Techniques and Applications

Under the auspices of the Protein Analysis Working Group (PAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) a key comparison, CCQM-K115, was coordinated by the Bureau International des Poids et Mesures (BIPM) and the Chinese National Institute of Metrology (NIM). Eight Metrology Institutes or Designated Institutes and the BIPM participated. Participants were required to assign the mass fraction of human C-peptide (hCP) present as the main component in the comparison sample for CCQM-K115. The comparison samples were prepared from synthetic human hCP purchased from a commercial supplier and used as provided without further treatment or purification. hCP was selected to be representative of the performance of a laboratory's measurement capability for the purity assignment of short (up to 5 kDa), non-cross-linked synthetic peptides/proteins. It was anticipated to provide an analytical measurement challenge representative for the value-assignment of compounds of broadly similar structural characteristics. The majority of participants used a peptide impurity corrected amino acid analysis (PICAA) approach as the amount of material that has been provided to each participant (25 mg) is insufficient to perform a full mass balance based characterization of the material by a participating laboratory. The coordinators, both the BIPM and the NIM, were the laboratories to use the mass balance approach as they had more material available. It was decided to propose KCRVs for ...