PPR-SMR protein SOT1 has RNA endonuclease activity

作者：Wen Zhou, Qingtao Lu, Qingwei Li, Lei Wang, Shunhua Ding, Aihong Zhang, Xiaogang Wen, Lixin Zhang, Congming Lu · 发表于：Proceedings of the National Academy of Sciences · 年份：2017 · DOI：10.1073/pnas.1612460114 · 被引用次数：90 · 研究领域：RNA modifications and cancer、RNA and protein synthesis mechanisms、Photosynthetic Processes and Mechanisms

pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5' end of the chloroplast 23S-4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.