Expression map of a complete set of gustatory receptor genes in chemosensory organs of Bombyx mori

作者：Huizhen Guo, Tingcai Cheng, Zhiwei Chen, Liang Jiang, Youbing Guo, Jianqiu Liu, Shenglong Li, Kiyoko Taniai, Kiyoshi Asaoka, Keiko Kadono‐Okuda, Kallare P. Arunkumar, Jiaqi Wu, Hirohisa Kishino, Huijie Zhang, Rakesh Kumar Seth, Karumathil P. Gopinathan, Nicolas Montagné, Emmanuelle Jacquin‐Joly, Marian R. Goldsmith, Qingyou Xia, Kazuei Mita · 发表于：Insect Biochemistry and Molecular Biology · 年份：2017 · DOI：10.1016/j.ibmb.2017.02.001 · 被引用次数：87 · 研究领域：Neurobiology and Insect Physiology Research、Invertebrate Immune Response Mechanisms、Insect and Arachnid Ecology and Behavior

Most lepidopteran species are herbivores, and interaction with host plants affects their gene expression and behavior as well as their genome evolution. Gustatory receptors (Grs) are expected to mediate host plant selection, feeding, oviposition and courtship behavior. However, due to their high diversity, sequence divergence and extremely low level of expression it has been difficult to identify precisely a complete set of Grs in Lepidoptera. By manual annotation and BAC sequencing, we improved annotation of 43 gene sequences compared with previously reported Gr s in the most studied lepidopteran model, the silkworm, Bombyx mori , and identified 7 new tandem copies of BmGr30 on chromosome 7, bringing the total number of BmGrs to 76. Among these, we mapped 68 genes to chromosomes in a newly constructed chromosome distribution map and 8 genes to scaffolds; we also found new evidence for large clusters of BmGrs , especially from the bitter receptor family. RNA-seq analysis of diverse BmGr expression patterns in chemosensory organs of larvae and adults enabled us to draw a precise organ specific map of BmGr expression. Interestingly, most of the clustered genes were expressed in the same tissues and more than half of the genes were expressed in larval maxillae, larval thoracic legs and adult legs. For example, BmGr63 showed high expression levels in all organs in both larval and adult stages. By contrast, some genes showed expression limited to specific developmental stages or o...