Advanced Non-Destructive Ocular Visualization Methods by Improved X-Ray Imaging Techniques

作者：Christian Enders, Eva-Maria Braig, K. Scherer, Jens Ulrich Werner, Gerhard Lang, Gabriele E. Lang, Franz Pfeiffer, Peter B. Noël, Ernst J. Rummeny, Julia Herzen · 发表于：PLoS ONE · 年份：2017 · DOI：10.1371/journal.pone.0170633 · 被引用次数：11 · 研究领域：Advanced X-ray Imaging Techniques、Photoacoustic and Ultrasonic Imaging、Cell Image Analysis Techniques

Due to limited X-ray contrast, the use of micro-CT in histology is so far not as widespread as predicted. While specific staining procedures-mostly using iodine-address this shortcoming, long diffusion times restrict its use in the often time-constrained daily routine. Recently, a novel staining protocol has been proposed using a biochemical preconditioning step, which increases the permeability of the cells for the staining agent. This could enable the imaging of entire organs of small mammals at a yet unmatched image quality with reasonable preparation and scan times. We here propose an adaptation of this technique for virtual ophthalmology and histology by volumetrically assessing both human and porcine eyes. Hereby, we demonstrate that (contrast-enhanced) micro-CT can outperform conventional histology in the assessment of tumor entities, as well as functioning as a supplementary tool for surgeons in the positioning of intraocular implants in-vitro and as a general assessment tool for ophthalmologic specimens.